Tag
SpyTag

Part:BBa_K3183013

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


SpyTag, Codon-Optimized for L. reuteri

This part (SpyTag) codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher.1 This part is codon-optimized for L. reuteri. That catcher protein for this oligopeptide can be found under BBa_K1159200. For more information on SpyTag, please see BBa_K1159201.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Oxford iGEM 2019

This part is used in our composite parts BBa_K3183103 and BBa_K3183300.

References

Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. (2012). Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 20;109(12)

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Categories
Parameters
None